... This is a continuation from message #14 ...
Uh Bart, what purpose is being defeated?? If you try to shoot a Pygmy Seahorse with a Subsee on a long lens, the DOF will be so narrow, his snout will be blurry if his eyes are in focus! Of couse the angular and spatial resolution are inversely proportional to the diameter of the aperture, but that only holds water in the infinitesimally small optimum focal plane. We are not benchmarking sensor resolution here, we are taking pictures in the real world!
My comment you quoted was a suggestion for making the use of the Subsee easier, it does not address the virtues of the use of the Subsee. Had you taken the time to read all of the posts in this thread, you would have seen my post to Tim, extoling the virtue of using a shorter lens and cropping when using the higher resolution cameras. It seems that you have missed the point entirely!
Hi Doug, I did read your comment on using the 60mm macro and fully agreed with it (even though Tim considers it cheating). I did not realise that that comment and the one on easing the use of the SubSee were both from you, but it doesn't really matter. The reason for my reply was that in discussions of supermacro the focus, stated or not, often centres on magnification instead of resolution. In practise the two are related, but they are not the same and in my opinion this becomes especially important for supermacro and current high-resolution sensors with very small pixels. The same issue was recently raised by Alex in his review of the Nikon D7100 and I've copied a revealing section below.
"The only time we may want to consider diffraction on the D7100 is when we are shooting super macro. If you never shoot super macro, no problem! If you do, you have two options. First, just ignore it and use the aperture you want for depth of field you desire. This approach is fine and ignoring diffraction will leave you with images, even at the smallest apertures that are as good as you could have got with a D7000. The alternative is to worry about diffraction and shoot super macro at more open apertures. While this will allow you to realize the full potential of your 24MP, it will mean you are deleting lots of images because of lack of depth of field.
I think the best solution is to generally pick the aperture you need, but only go above f/32 in extreme cases. Ultimately it is the image that matters and it won’t in any way be ruined by a bit of diffraction, you simply loose the extra resolution advantage and end up with an image you could have taken with the 16MP D7000 – hardly the end of the world."
Alex is clearly aware of the interplay between DOF and resolution, as are you and most others, but I come to a somewhat different conclusion. The difference is subtle and not so important until you get into supermacro. One way to think about it relates to how you define supermacro. It is often stated as "a magnification larger than 1:1" but in my opinion it is better to think of it as "resolving details smaller than the resolution limit of the sensor". To do the latter you need a magnification larger than 1:1 but a magnification larger than 1:1 does not necessarily give you higher resolution. Another way to think about it, and more closely related to how we shoot, is as follows.
Start with your subject of interest. In this case let's stick with the pygmy seahorse. Given the shape and dimensions of its head you need a certain amount of DOF to get both eye and snout in focus. That amount of DOF directly sets the maximum resolution you can obtain. If this resolution is already less than what the sensor can resolve then you are not doing supermacro. Adding diopters, extension tubes, or teleconverters does not change that and in general will just make taking the image more challenging. As you and Alex said; getting the desired DOF takes precedence, you care about "the shot" not "benchmark the sensor". But the point I was hoping to make is that it is worthwhile to consider whether the DOF requirements for your subject qualify it for supermacro photography. If not, taking the diopter off is likely preferable to closing the aperture and boosting ISO.
Bart